Abstract
The specificity of the 19-OH F 1α F 2α prostaglandin for the detection of semen in seminal/vaginal mixtures, has been evaluated. Using a parallel curve test we found that the antibody showed a high specificity for these seminal prostaglandins in seminal/vaginal mixtures at concentrations of between 2 pg and 40 pg/100 μl. The precision of the assay has been improved by the use of a donkey-anti-rabbit ferritinbound second antibody. The application of this detection system makes it possible to complete an assay within 4.5 h. A survey of 50 semen-free vaginal swabs obtained from 3 donors, taken throughout the menstrual cycle, showed no trace of these prostaglandins. They were also not detected in the vaginal secretions of two further donors who were undergoing medication. Using only 10-μl aliquots of a seminal/vaginal swab extract, prepared in 500 μl, it was possible to detect semen up to approx. 80 h after one sexual act.
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