The application of a sensitive uricase-peroxidase coupled reaction to a centrifugal fast analyser for the determination of uric acid

Abstract

A uricase-peroxidase coupled system, for the determination of uric acid, applied to a CentrifiChem-500 centrifugal fast analyser is described. Relatively large amounts of ascorbic acid, due to the inclusion of an ascorbate oxidase urine diluent, and hemoglobin appear not to interfere with the procedure while the incorporation of potassium ferrocyanide into the reagents has led to the near-total elimination of bilirubin interference. The incubation period is relatively short compared with other similar procedures and the one reagent system has made the procedure simple to perform. The use of sodium 2-hydroxy-3,5-dichlorobenzenesulfonate and 4-aminoantipyrene in the oxidative coupling reaction has incorporated the advantages of increased sensitivity, over phenol-4-aminoantipyrene systems, as well as the amenability of the reagent towards lyophilization or “dry-fill”.