Abstract
The Δe-method of analysis, which was developed for the investigation of lignins, involves the selective modification of a given chromophore in a mixture of chromophores. This method permits the quantitative determination of a single ultraviolet-absorbing compound in mixtures of such absorbing materials. The analyses of eugenol in clove oil, in eugenyl acetate, and in two formulations are described as illustrations of the pharmaceutical application of this technique. The absorbance at 296 m μ of an aliquot of the eugenol-containing preparation was determined in basic solution. The change in absorbance was then compared to the bathochromic displacement of a standard eugenol solution with a corresponding change in pH.
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