Abstract
Optical imaging is a popular method for biology research, and now there lots of optical imaging system. Confocal imaging is a wide used one which was introduced the pinhole and subsequently is not affected by the out-of-focus signal, making the method be better than widefield microscopy. It is acceptable that confocal microscopy has its own limits in resolution. Researchers struggled to push the resolution to another limit during the process many fancy optical imaging methods were invented, such as structured illumination microscopy (SIM). We are here to apply the two optical imaging methods in RyR2 labelled heart cells to compare the two methods, and we found that confocal imaging does show the disadvantages such as photobleaching and limited resolution, while SIM imaging has a higher resolution to observe much more details of the RyR2 location.
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