Abstract
In studies conducted by the Department of Plant Pathology of the Agricultural Research Centre, soil-borne viruses were found in soil samples from seven nurseries out of thirteen sampled and from fields at two experimental stations in south and south-west Finland. Tobacco necrosis virus was identified for the first time in Finland in 12 isolates and in addition tobacco rattle virus in 8 isolates. All these isolates were made from 193 soil and plant samples collected in late autumn of 1970. For the first time in Finland the nematode Trichodorus pachydermus Seinhorst was identified from extracts of TRV-infected soil and plant samples.
Highlights
In studies conducted by the Department of Plant Pathology of the Agricultural Research Centre, soil-borne viruses were found in soil samples from seven nurseries out of thirteen sampled and from fields at two experimental stations in south and south-west Finland
Late in the autumn of 1970, when the ground was partly frozen, 193 soil samples were collected from thirteen nurseries in south and south-west Finland and from the experimental fields of the Agricultural Research Centre at Tikkurila and of the Department of Horticulture at Piikkiö
21 viruses were isolated from samples originating from seven of the thirteen nurseries and from both experimental stations
Summary
Late in the autumn of 1970, when the ground was partly frozen, 193 soil samples were collected from thirteen nurseries in south and south-west Finland and from the experimental fields of the Agricultural Research Centre at Tikkurila and of the Department of Horticulture at Piikkiö. Weeds were present in 43 of these samples and in 17, perennial plants with roots. The roots of the weeds and perennials were macerated in phosphate buffer, and leaves of Chenopodium quinoa Willd. Samsun were inoculated with the resultant suspension. Samsun tobacco seedlings were planted as »bait plants» in soil samples which were put into 5’ pots. After two weeks sap from their roots was used to inoculate C. quinoa and N. tabacum. The experiments were continued with all samples which caused primary and/or secondary symptoms on the above-mentioned test plants. The thermal end point was determined for all the virus isolates and the dilution end point and ageing in vitro for some of these
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