Abstract
The solute carrier family 26 (SLC26) gene family encodes at least 10 different anion exchangers. SLC26 member 6 (SLC26A6 or CFEX/PAT-1) and the cystic fibrosis transmembrane conductance regulator (CFTR) co-localize to the apical membrane of pancreatic duct cells, where they act in concert to drive HCO3- and fluid secretion. In contrast, in the small intestine, SLC26A6 serves as the major pathway for oxalate secretion. However, little is known about the function of Slc26a6 in murine salivary glands. Here, RNA sequencing-based transcriptional profiling and Western blots revealed that Slc26a6 is highly expressed in mouse submandibular and sublingual salivary glands. Slc26a6 localized to the apical membrane of salivary gland acinar cells with no detectable immunostaining in the ducts. CHO-K1 cells transfected with mouse Slc26a6 exchanged Cl- for oxalate and HCO3-, whereas two other anion exchangers known to be expressed in salivary gland acinar cells, Slc4a4 and Slc4a9, mediated little, if any, Cl-/oxalate exchange. Of note, both Cl-/oxalate exchange and Cl-/HCO3- exchange were significantly reduced in acinar cells isolated from the submandibular glands of Slc26a6-/- mice. Oxalate secretion in submandibular saliva also decreased significantly in Slc26a6-/- mice, but HCO3- secretion was unaffected. Taken together, our findings indicate that Slc26a6 is located at the apical membrane of salivary gland acinar cells, where it mediates Cl-/oxalate exchange and plays a critical role in the secretion of oxalate into saliva.
Highlights
The solute carrier family 26 (SLC26) gene family encodes at least 10 different anion exchangers
CHO-K1 cells transfected with mouse Slc26a6 exchanged Cl؊ for oxalate and HCO3؊, whereas two other anion exchangers known to be expressed in salivary gland acinar cells, Slc4a4 and Slc4a9, mediated little, if any, Cl؊/oxalate exchange
These results show that Slc26a6 does not support fluid secretion but that Slc26a6 significantly contributes to oxalate secretion in the intact mouse SMG organ system
Summary
The solute carrier family 26 (SLC26) gene family encodes at least 10 different anion exchangers. CHO-K1 cells transfected with mouse Slc26a6 exchanged Cl؊ for oxalate and HCO3؊, whereas two other anion exchangers known to be expressed in salivary gland acinar cells, Slc4a4 and Slc4a9, mediated little, if any, Cl؊/oxalate exchange. Our findings indicate that Slc26a6 is located at the apical membrane of salivary gland acinar cells, where it mediates Cl؊/oxalate exchange and plays a critical role in the secretion of oxalate into saliva. Targeted disruption of mouse Slc26a6 inhibited HCO3Ϫ and fluid secretion in the pancreas [13], whereas oxalate secretion in the small intestine and reabsorption by the kidney were markedly reduced (14 –17).
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