The antiurolithiasic and hepatocurative activities of aqueous extracts of Petroselinum sativum on ethylene glycol-induced kidney calculi in rats

Abstract

Forty-eight (48) male Wistar rats were randomly assigned into 6 groups and treated for 30 days. Group A served as normal control and group B ethylene glycol (EG) control received 1% EG in drinking water. Groups C, D, E and F received 1% EG from day 0 and were used as the treatment subjects. Rats in groups C and D received 200 and 600 mg/kg body weight (BW) of aerial parts aqueous extract, respectively and those in groups E and F received 200 and 600 mg/kg BW of root aqueous extract in drinking water, respectively from day 14 of the experiment. The blood samples were collected on days 0 and 30, and serum level of creatinine, uric acid, urea, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity were evaluated. In day 31, the kidneys were removed and prepared for histologic evaluation of calcium oxalate (CaOx) deposits. The concentrations of serum urea, uric acid and creatinine were increased (P<0.05) in group B compared with control group and were decreased (P<0.05) in treatment groups (C, D, E and F) compared with group B. The values of ALT was decreased (P<0.05) in groups C, D and E and the values of AST was decreased (P<0.05) just in groups C and E compared with group B. The number of CaOx deposits in group B was higher (P<0.05) than that in group A and in treatment groups C, D, E and F were decreased (P<0.05 compared with group B. The presented data indicate that the aqueous extract of Petroselinum sativumreduced the growth of urinary stones and hepatotxicity induction by EG in rats. We suggest further studies to establish the mechanism of toxicity or pharmacological effects of P. sativum in human. Key words: Petroselinum sativum, kidney stone, alanine aminotransferase, calcium oxalate, aspartate aminotransferase.