Abstract
Juglans mandshurica Maxim is a traditional herbal medicines in China, and its anti-tumor bioactivities are of research interest. Bioassay-guided fractionation method was employed to isolate anti-tumor compounds from the stem barks of the Juglans mandshurica Maxim. The anti-tumor effect and biological activities of the extracted compound JMM6 were studied in BEL-7402 cells by MTT, Cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay and Detection of mitochondrial membrane potential (ΔΨm). After treatment with the JMM6, the growth of BEL-7402 cells was inhibited and cells displayed typical morphological apoptotic characteristics. Further investigations revealed that treatment with JMM6 mainly caused G2/M cell cycle arrest and induced apoptosis in BEL-7402 cells. To evaluate the alteration of mitochondria in JMM6 induced apoptosis. The data showed that JMM6 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane. Our results show that the JMM6 will have a potential advantage of anti-tumor, less harmful to normal cells. This paper not only summarized the JMM6 pick-up technology from Juglans mandshurica Maxim and biological characteristic, but also may provide further evidence to exploit the potential medicine compounds from the stem-barks of the Chinese Juglans mandshurica Maxim.
Highlights
Traditional Chinese medicine-based herbal medicines have gained more and more attention in the recent years and are being pursued by pharmaceutical companies as rich sources for drug discovery (Hsiao and Liu, 2010)
Little attention has been paid to screen the main extracts by Bioassay-guided fractionation and search their bioactivities, especially, the anti-tumor biological mechanism of the medicinal compound from the Chinese Juglans mandshurica Maxim stem-barks
The stem-barks of Juglans mandshurica Maxim were collected from Yiliga Mountain, Heilongjiang Province, China, dried at room temperature for 3 weeks
Summary
Traditional Chinese medicine-based herbal medicines have gained more and more attention in the recent years and are being pursued by pharmaceutical companies as rich sources for drug discovery (Hsiao and Liu, 2010). Juglans mandshurica Maxim is a traditional herbal medicine in China. Roots, stem-barks and green husks have been used as a traditional herbal medicine to treat or prevent various diseases (Kim et al, 1998; Lee et al, 2002; Lee et al, 2005; Reutrakul et al, 2006). Many components such as quinones, flavones, polyphenols, glycosides, volatile oils have been paid attention in this plant (Machida et al, 2009; Li et al, 2009; Liu et al, 2004). Little attention has been paid to screen the main extracts by Bioassay-guided fractionation and search their bioactivities, especially, the anti-tumor biological mechanism of the medicinal compound from the Chinese Juglans mandshurica Maxim stem-barks
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