The Antistaphylococcal Activity of Citropin 1.1 and Temporin A against Planktonic Cells and Biofilms Formed by Isolates from Patients with Atopic Dermatitis: An Assessment of Their Potential to Induce Microbial Resistance Compared to Conventional Antimicrobials.

Malgorzata Dawgul,Wioletta Baranska-Rybak,Roman Nowicki,Damian Neubauer,Marta Bauer,Lidia Piechowicz,Wojciech Kamysz

doi:10.3390/ph9020030

Malgorzata Dawgul, Wioletta Baranska-Rybak + Show 5 more

Open Access

https://doi.org/10.3390/ph9020030

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Journal: Pharmaceuticals	Publication Date: May 25, 2016
Citations: 58	License type: CC BY 4.0

Affiliation: Gdańsk Medical University

Abstract

Staphylococcus aureus (SA) colonizes the vast majority of patients with atopic dermatitis (AD). Its resistance to antibiotics and ability to form biofilms are the main origins of therapeutic complications. Endogenous antimicrobial peptides (AMPs) exhibit strong activity against SA, including antibiotic resistant strains as well as bacteria existing in biofilm form. The purpose of the present work was to determine the antistaphylococcal activity of two amphibian peptides against SA isolated from patients with AD. The AMPs demonstrated permanent activity towards strains exposed to sublethal concentrations of the compounds and significantly stronger antibiofilm activity in comparison to that of conventional antimicrobials. The results suggest the potential application of amphibian AMPs as promising antistaphylococcal agents for the management of skin infections.

Highlights

The skin serves as the first line of defense against infection by reducing microbial adherence and invasion [1]
The antimicrobial peptides (AMPs) exhibited activity against all Staphylococcus aureus (SA) strains isolated from patients with atopic dermatitis (AD), while several strains were resistant to conventional antistaphylococcal agents
The activity of antibiotics against the majority of the isolates in their planktonic form was significantly higher in comparison to that of the AMPs

Summary

Introduction

The skin serves as the first line of defense against infection by reducing microbial adherence and invasion [1]. In atopic dermatitis (AD), a chronic, relapsing inflammatory skin disease, the protective epidermal barrier is significantly compromised [2,3]. Both the etiology and pathogenesis of AD are very complex in nature. SA is the major cause of skin and soft tissue infections which, despite the continuous development of medicines, are still difficult to treat [5,6]. Therapeutic problems are caused by the development of strains with reduced susceptibility to many antibiotics, such as methicillin-resistant SA (MRSA) [7]

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