Abstract
The effect of LiCl on melanoma cell growth and differentiation was studied in mouse and human melanoma cell lines. LiCl markedly inhibited B16 and HT-144 melanoma cell growth in vitro. Clonogenicity in soft agar of the melanoma cells was also markedly inhibited by LiCl. Pretreatment of B16 mouse melanoma cells with LiCl delayed the appearance of melanoma tumours in syngeneic mice. Growth inhibition of cells was accompanied by morphological and biochemical alterations. LiCl induced cell enlargement and formation of dendrite-like structures. The activity of NADPH cytochrome c reductase, an enzymatic marker of endoplasmic reticulum was significantly (2-3 fold) increased. Addition of myo-inositol to cell cultures partially reversed the anti-proliferative and morphological effects of LiCl on melanoma cells. This finding may suggest that the anti-proliferative effect of LiCl is related to its effect on phosphatidylinositol metabolism.
Highlights
We demonstrate that lithium chloride (LiCI) inhibits melanoma cell growth and clonogenicity and delays the appearance of tumours in syngeneic mice
Forty-eight hours of LiCI (5 mM) treatment resulted in a decrease in B16 FIO melanoma cell proliferation of about 50% (Figure IA)
We considered the possibility that the action of LiCI on melanoma cells might be related to an effect on phosphatidylinositol metabolism
Summary
A melanotic clone of mouse B16 melanoma FIO, kindly donated by Dr Abraham Raz, the Weizmann Institute, was Medical College, New York, NY, USA. Received January 28 1986; and in revised from. In selected experiments a human amelanotic melanoma cell line HT-144 from the American Type Culture Collection, Maryland was used. The cells were cultured in RPMI 1640, supplemented with 10% foetal calf serum and antibiotics, at 37'C in a humidified atmosphere of 5% CO2 and 95% air. B16 FIO cells were passaged three times weekly and HT-144 cells 1-2 times weekly. Media and supplements were obtained from Biol. Industries, Israel and lithium chloride from BDH chemicals, Ltd., Poole, UK
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