The antioxidative detection of Prdx6 to cell membrane lipid peroxidation of lung type II epithelial cells by DPPP (693.3)

Abstract

Peroxiredoxin 6 (Prdx6) is a peroxidase antioxidant enzyme, we observed the protection of Prdx6 to cell membrane lipid peroxidation of lung alveolar epithelial typeIIcells (AEC II) by using a novel fluorescent probe Diphenyl‐1‐pyrenylphosphine (DPPP). Hydrogen peroxide (H2O2) can diffuse across cell membrane, so the dose of 500 µM H2O2 was used to AEC II exposure with labeled DPPP at 400nmol/m for 1 to2 h, the AECIIwere isolated from wild type (WT) and Prdx6 transgenic overexpressor (Tg) mice and observed under fluorescence microscopy and PTI spectrofluorometer. We found that AECIIwith H2O2 exposure induced time‐related fluorescence signal increasing in cell membrane under fluorescence microscopy, and the AECIIfrom Prdx6 Tg mice showed about 36% less cell membrane lipid peroxidation compared to WT group; under PTI spectrofluorometer study, the decreased cell membrane phospholipid peroxidation in Tg Prdx6 AECIIconsistently presented about 60% decreasing remarkably in 2h‐ H2O2 exposure and about 30% in 1h‐ H2O2 exposure compared to WT group. Thus, transgenic overexpression of Prdx6 in AECII defends from H2O2‐induced cell membrane lipid peroxidation.Grant Funding Source: Heart and Lung Society