The Antioxidant Phytochemical Schisandrin A Promotes Neural Cell Proliferation and Differentiation after Ischemic Brain Injury.

Wentian Zong,Weijie Xu,Ruofeng Wang,Paulo E S Munekata,Enli Cai,Yuming Wu,Mostafa Gouda,José M Lorenzo

doi:10.3390/molecules26247466

Abstract

Schisandrin A (SCH) is a natural bioactive phytonutrient that belongs to the lignan derivatives found in Schisandra chinensis fruit. This study aims to investigate the impact of SCH on promoting neural progenitor cell (NPC) regeneration for avoiding stroke ischemic injury. The promoting effect of SCH on NPCs was evaluated by photothrombotic model, immunofluorescence, cell line culture of NPCs, and Western blot assay. The results showed that neuron-specific class III beta-tubulin (Tuj1) was positive with Map2 positive nerve fibers in the ischemic area after using SCH. In addition, Nestin and SOX2 positive NPCs were significantly (p < 0.05) increased in the penumbra and core. Further analysis identified that SCH can regulate the expression level of cell division control protein 42 (Cdc42). In conclusion, our findings suggest that SCH enhanced NPCs proliferation and differentiation possible by Cdc42 to regulated cytoskeletal rearrangement and polarization of cells, which provides new hope for the late recovery of stroke.

Highlights

Stroke is a very serious disabling disease, and there is no effective therapeutic strategy at present for this disorder [1]
We evaluated the capacity of Schisandrin A (SCH) to promote neural progenitor cell (NPC) proliferation and differentiation, through activation of brain cells and neurological functional recovery after stroke
Neural Progenitor Cell (NPC) Regeneration after Cerebral Ischemia Caused by Photothrombosis

Summary

Introduction

Stroke is a very serious disabling disease, and there is no effective therapeutic strategy at present for this disorder [1]. There are still questions coming along with neuronal regeneration after such ischemia severe of stroke [3]. In this context, NPCs are one of the best ways to enhance the recovery of these kinds of diseases. NPCs are self-renewing and multipotent cells that generate three major cell types in the brain called oligodendrocytes, neurons, and astrocytes [4]. NPCs proliferate and generate new neurons to compensate for cell damage. Transplantation of NPCs to treat stroke still needs a lot of in-depth research further and the most feasible method to enhance neuronal repair is promoting endogenous NPCs proliferation migration [5,6]

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