The Antioxidant and Hepatoprotective Potential of Temulawak (Curcuma xanthorrhiza Roxb) Ethanol Extract in Paracetamol-induced Rats

Abstract

Paracetamol is widely used as analgesic and antipyretic drugs. Paracetamol can increase Radical Oxygen Species (ROS) if used exceeded the administered dose that might lead to hepatic and gastric damage. The purpose of this research is to investigate the antioxidant and hepatoprotective potential of Temulawak Ethanol Extract in Paracetamol-induced rats. This research was conducted using completely randomized design. The samples were 30 white rats, divided equally into 6 groups. The rats of group 1 were fed normally with regular feed and aquadest for 10 days (control). The rats of group 2 were fed using aquadest and temulawak extract with the dosage of 540mg/200g rats body weight for 10 days. The group 3 of rats were fed using aquadest and paracetamol with the dosage of 2g/kg rats body weight on the 7th day (positive control). The group 4,5, and 6 were fed using Temulawak ethanol extract, 270mg/200g, 540mg/200g, and 1080mg/200g rats body weight, respectively, for 10 days and were given 2g/kg rats body weight of paracetamol on 7th day. The rats blood samples were taken after 10 days treatment. The MDA, ALT and AST in rats blood serum were measured. The data were analyzed using ANOVA and LSD test. The treatment of 270mg/200g, 540mg/200g, and 1080mg/200g rats body weight Temulawak ethanol extract showed specific results of lowered ALT, ASP and MDA levels in paracetamol induced rats. It can be concluded from this research that the Temulawak ethanol extract is cosidered potential as antioxidant and hepatoprotector.