The antioxidant and cytotoxic properties of Morinda citrifolia and Morinda elliptica leaves extracts

Abstract

Event Abstract Back to Event The antioxidant and cytotoxic properties of Morinda citrifolia and Morinda elliptica leaves extracts Nurulaidah Esa1 and Latifah Saiful Yazan1* 1 Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, department of biomedical science, Malaysia Background Cancer is one of the top ten causes of hospitalisation and death in Malaysian hospitals. Standard treatments for cancer patients include surgery, chemotherapy and radiotherapy, but with several adverse effects. Hence, researchers have shifted their focus on natural resources as potential treatment. Morinda citrifolia and M. elliptica of the Rubiaceae family have been traditionally used to treat diabetes, gastric, ulcer, inflammation and cancer. The plants were found to exert these promising therapeutic effects given their antioxidant properties. This study investigated the antioxidant and cytotoxic properties of M. citrifolia and M. elliptica leaves extracts. Methodology Morinda ethanol extracts were prepared by immersing the leaves for one day in 50, 70 or 100% ethanol (1:10, w/v) at room temperature while the aqueous extracts were boiled in distilled water for 0, 5, 15, 30 and 60 minutes (1:100, w/v). The extracts were subjected to antioxidant assays - total phenolic content (TPC), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay, 2,2-azinobis-3-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and ferric ion reducing antioxidant power (FRAP). The cytotoxicity of the extracts on human colorectal adenocarcinoma cell line (HT-29) were determined using MTT assay. Results The M. elliptica aqueous extract has the highest antioxidant activities among all the extracts. M. elliptica, boiled for 30 minutes has the highest TPC (75.98+4.53 mg GAE/g extract) and while at 0 minute, it has the highest FRAP (176.33+4.4 TEAC/g extract). M. elliptica has the highest DPPH (18.22+0.62 mg TEAC/g extract) and ABTS (219.84+2.93 mg TEAC/g extract) radical scavenging activity when boiled for 5 minutes. The M. elliptica extract in 100% ethanol was the most cytotoxic among all the extracts with IC50 of 105.5 + 1.0 µg/mL at 72 hours. Conclusion The M. elliptica leaves extracts have better antioxidant and cytotoxic properties when compared to the M. citrifolia leaves extracts. Acknowledgements I would like to express my utmost gratitude to my supervisor Associate Professor Dr Latifah Saiful Yazan and co- supervisors; Professor Dr. Suhaila Mohamed, Associate Professor Dr. Mohamad Aris Mohd Moklas, Associate Professor Dr. Zainul Amiruddin Zakaria and Dr. Razana Mohd Ali for their generous input and constructive criticism for this project. Keywords: Morinda citrifolia, Morinda elliptica, antioxidant, cytotoxic, HT-29 Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Cancer Citation: Esa N and Saiful Yazan L (2019). The antioxidant and cytotoxic properties of Morinda citrifolia and Morinda elliptica leaves extracts. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00131 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2018; Published Online: 17 Jan 2019. * Correspondence: Prof. Latifah Saiful Yazan, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, department of biomedical science, Serdang, Selangor, 43400, Malaysia, latifahsy@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nurulaidah Esa Latifah Saiful Yazan Google Nurulaidah Esa Latifah Saiful Yazan Google Scholar Nurulaidah Esa Latifah Saiful Yazan PubMed Nurulaidah Esa Latifah Saiful Yazan Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.