Abstract
The aim of this work was to provide a preliminary characterization of alkalic-extractable polysaccharides (ALPS) from Coprinus comatus, to explore its in vivo antioxidant activities and protective effects on alcohol-induced liver injury. ALPS showed strong antioxidant and anti-inflammatory abilities and markedly low serum enzyme activities, hepatic and serum lipid levels, as well as low hepatic lipid peroxidation levels; moreover, ALPS improved the alcohol metabolism system. These results were also confirmed by an analysis of histopathological section observations. ALPS, in both α- and β-configurations, as analysed by fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR), was mainly composed of rhamnose (Rha), fucose (Fuc), ribose (Rib), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glu) with mass percentages of 0.52%, 1.02%, 0.80%, 0.92%, 3.05%, 2.96% and 90.73%, respectively. These results may offer support for the use of ALPS as a functional food or natural drug source that can prevent and treat alcohol-induced liver injury.
Highlights
Alcoholic liver disease (ALD) has become a major cause of morbidity and mortality all over the world[1]
The scavenging activities of alkalic-extractable polysaccharides (ALPS) and butylated hydroxytoluene (BHT) on hydroxyl radicals were shown in Fig. 1B and a concentrationdependent effect was observed
We determined that ALPS can effectively improve dyslipidaemia by negative regulation on low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) levels and positive regulation on high-density lipoprotein cholesterol (HDL-C) level, alleviate hepatic lipid metabolism disorder by reducing hepatic TC and TG, mitigate oxidative stress via enhancing antioxidant enzyme activities and decreasing lipid peroxide products and improve effects from alcohol metabolism disorder by suppressing cytochrome P4502E1 (CYP2E1) activity in mice with acute alcohol-induced hepatic injury
Summary
Alcoholic liver disease (ALD) has become a major cause of morbidity and mortality all over the world[1]. The exploration of effective treatment for ALD and the use of agents for protecting against liver injury induced by alcohol have drawn more and more attention. Antioxidants from natural products have garnered great attention for their potential function in prevention and treatment of alcohol-induced liver injury[7]. Natural products from the fruiting body, mycelia and spent mushroom substrate, have been verified to have many pharmacological abilities such as antioxidant, hepatoprotective, hypolipidaemic and anti-diabetic activities[8,9,10]. Comatus mainly focused on biological activity assays of the water-extractable fruiting body and mycelia polysaccharides. The objective of this work was to provide a preliminary characterization of ALPS, to investigate the antioxidant in vitro and in vivo and hepatoprotective activities of ALPS on acute alcohol-induced liver injury in mice
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
