Abstract
The hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus), as an important economic fish, has attracted much attention for its meat quality, and its meat quality is closely related to the composition of diet. In this study, a rearing experiment was conducted for 65 days with diets containing 0, 3, 6, and 9% oxidized fish oil (referred to as R, L, M and H group respectively) to investigate the effects of oxidized fish oil on the antioxidant ability, histology, proximate, amino acid and fatty acid compositions, and transcriptome analysis of muscle in juvenile hybrid grouper. As the proportion of oxidized fish oil increased in diet, the vitamin E concentration and antioxidant enzymes activities decreased significantly in muscle, but malondialdehyde (MDA) and total antioxidant capacity (T-AOC) showed the opposite trend, and the destruction of muscle fiber by oxidative stress was increasing. At the same time, the proximate composition, amino acid and fatty acid content in muscle were affected by oxidative stress. A total of 43,469 unigenes were obtained in transcriptome with 76 differentially expressed genes. There were 3 significant pathways including vitamin B6 metabolism, biosynthesis of amino acids, and p53 signaling pathway, and the significant pathways involved 5 different genes including phosphatase, phosphoglycerate mutase 2 (PGAM2), S-adenosylmethionine synthase isoform type-2 (SAM2), protein shisa-5-like (SHISA5), and growth arrest and DNA damage-inducible protein GADD45 beta-like (GADD45β). In conclusion, the dietary oxidized fish oil caused oxidative stress of muscle, and oxidative stress destroyed muscle fiber structure. Meanwhile, the oxidative stress changed muscle nutrition. In addition, the oxidative stress changed the muscle transcriptome profile.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.