Abstract

Electrogenesis linked to cyclic electron transfer through the ubiquinol-cytochrome 5c 2 oxidoreductase complex in Rhodopseudomonas sphaeroides chromatophores was investigated by a direct electrometric method. Rapid kinetic measurements of laser flash-induced ΔΨ generated in chromatophores associated with a phospholipid-impregnated collodion film revealed an exponential rise ofΔΨ with a τ of ~ 700 μs. This exponential electrogenic phase contributes about 11% of the total photoelectric response and is sensitive to antimycin.

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