Abstract
The aim of the present study was to compare the antibacterial effectiveness of chlorhexidine and PPE oral rinse on S. mutans, Lactobacilli, and Veillonella, in clinical salivary samples of patients with advanced stages of dental caries at baseline and two and four weeks with PCR technique. This triple-blind randomized clinical trial involved 60 high caries risk adult patients, 19–59 years of age, randomly allocated into two groups of 30 subjects each. The intervention group received pomegranate peel extract mouthwash, whereas the control group received chlorhexidine mouthwash. Unstimulated pooled saliva was collected from the floor of the mouth before and after the intervention. The quantitative real-time polymerase chain reaction was employed to analyze the bacterial copies of each salivary sample at baseline and two and four weeks. The significance level was fixed at 5% (α = 0.05). Overall comparison of antimicrobial effectiveness across both groups revealed insignificant outcomes. The control group evinced a significant reduction in S. mutans between a specific time, i.e., baseline and 4 weeks (p=0.043). PPE oral rinse as a natural product or ecological alternative was effective in disrupting activity across all microorganisms tested in this triple-blind RCT; however, the nutraceutical, when compared to chlorhexidine, was not as effective against S. mutans.
Highlights
The use of chlorhexidine (CHX) and fluorides in prevention and treatment strategies has been directed at caries control and progression [1]
All things are considered; the aim of the present study was to compare the antibacterial effectiveness of chlorhexidine and Pomegranate Peel Extract (PPE) oral rinse on S. mutans, Lactobacilli, and Veillonella in clinical salivary samples of patients with advanced stages of dental caries at baseline (T0), two weeks (T1), and four weeks (T2) by employing a molecular technique, the quantitative real-time polymerase chain reaction
Study Design. e present study was performed as a triple-blind randomized clinical trial (RCT) wherein the primary investigator, participants, and the data analyzer were oblivious to the treatment or intervention being rendered
Summary
The use of chlorhexidine (CHX) and fluorides in prevention and treatment strategies has been directed at caries control and progression [1]. [4] A noninvasive ecological stratagem, propelling natural products into the cariological verse in a bid to deliver potentially active cariostatic catalytic agents in the form of mouthwashes, gels, varnishes, or chewing gums have been adopted, researched, and enforced in order to proselytize cariogenic microbial demise [5, 6]. In vitro experiments are plenteous, in vivo studies associating the comparison of the antibacterial efficacy of either Pomegranate Peel Extract (PPE) or other natural product oral rinses and CHX with. All things are considered; the aim of the present study was to compare the antibacterial effectiveness of chlorhexidine and PPE oral rinse on S. mutans, Lactobacilli, and Veillonella in clinical salivary samples of patients with advanced stages of dental caries at baseline (T0), two weeks (T1), and four weeks (T2) by employing a molecular technique, the quantitative real-time polymerase chain reaction (qPCR). A molecular approach (qPCR) was employed in this study on account of persistent reports of high sensitivity and specificity in the detection and quantification of microorganisms [11, 12]
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