Abstract
AbstractThis research was to study the effect of Melissa officinalis L. essential oil (MOEO) against Shewanella putrefaciens. The minimum inhibitory concentration and the growth curve indicated that MOEO could inhibit the growth of S. putrefaciens at a relatively low concentration (1 μl·ml−1). The increased values of glucose content, nucleic acid and protein showed that MOEO destroyed the S. putrefaciens cell membrane. Besides, the morphological and ultrastructural changes observed by scanning electron microscopy and transmission electron microscopy indicated that the S. putrefaciens cell membrane was destroyed irreversibly by MOEO. Meanwhile, the 0.5 μl·ml−1 MOEO could inhibit the formation and motility of biofilm as well as the production of extracellular polysaccharide. In general, MOEO destroyed the cell membrane to inhibit the growth of S. putrefaciens, which indicated MOEO had great potential to be used as a natural bacteriostat.
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