Abstract
Ozone is a known oxidant present in the atmosphere and is commercially produced by simple ozonizer machines. It is a powerful antimicrobial agent in its gaseous and aqueous forms. Ozone readily dissolves in water and retains its antimicrobial property even in the dissolved state. In this study, the effect of 0.1 ppm ozonated water was analyzed on 24-hour supragingival plaque (SP) samples in situ. SP was collected from the two most posterior teeth in the contra-lateral quadrants before and after a 30-second rinse with either distilled water (control group) or 0.1 ppm ozonated water (test group). The plaque was used to count the number of total bacteria, total anaerobic bacteria, Streptococcus mutans, and Candida albicans on selective agar media. The statistical analysis of the number of colony forming units (CFUs) obtained demonstrated a significant antimicrobial effect of ozonated water on the total bacteria (p = 0.01) and anaerobes (p = 0.02). A reduction in the post-rinse CFU count for Streptococcus mutans was also observed, but the effect was not statistically significant (p = 0.07). The Candida species was only grown from one sample. Ozonated water at the 0.1 ppm concentration was effective in reducing the load of 24-hour plaque bacteria, but it did not eliminate them completely.
Highlights
Bacteria in a biofilm are more resistant to antimicrobial agents[1] because their organized nature enables them to behave as a quorum
The main objective of this study was to determine the in situ antimicrobial effect of 0.1 ppm ozonated water on 24-hour plaque microorganisms following a 30-second rinse
The post-rinse colony forming units (CFUs) counts for total bacteria, anaerobes and the Streptococcus species showed a dif
Summary
Bacteria in a biofilm are more resistant to antimicrobial agents[1] because their organized nature enables them to behave as a quorum. Ozone (O3) in a gaseous or aqueous phase has been shown to be a powerful and reliable antimicrobial agent against bacteria, fungi, protozoa, and viruses.[9] It is an unstable gas capable of oxidizing any biological entity.[10] Its oxidative capacity at 100 ppm, 200 ppm and 400 ppm can induce serious toxicity due to lipid peroxidation and cause DNA damage.[11] A low concentration of ozonated water is sufficient
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