Abstract
In our previous study, we have found increased serum levels of HMGB1 in patients with Henoch– Schonlein purpura (HSP), allergic vasculitis (AV), and urticarial vasculitis (UV) and altered HMGB1 distribution in lesional skin in patients with HSP. HMGB1 plays a pro-inflammatory role in the pathogenesis of HSP. To further investigate the role of HMGB1 in the pathogenic mechanism of vasculitis, we investigated the anti-inflammatory effects of HMGB1 blockades (including anti-HMGB1 mAb and glycyrrhizin) in a mouse model of a cutaneous reverse passive Arthus (RPA) reaction. A total of 36 balb/c mice were randomly divided into four groups: the control group, IC model group, HMGB1 monoclonal antibody (anti-HMGB1-mAb) group and the glycyrrhizin group, with nine mice in each group. A cutaneous RPA reaction mouse model was established by injections of the OVA antibody and the OVA antigen. Mice of the anti-HMGB1-mAb group and glycyrrhizin group were pre-treated with anti-HMGB1 mAb or glycyrrhizin, respectively, before the RPA reaction. Our results indicated that HMGB1 blockades (anti-HMGB1 mAb and glycyrrhizin) obviously extenuated the severity of vasculitis skin damage and improved the histological evolvement of inflammatory cells infiltration, vascular fibroid necrosis, and vasodilation in a cutaneous RPA reaction mouse model. In addition, HMGB1 blockades reduced the infiltration of neutrophils, DCs, and T cells and decreased the mRNA expression of IL-6 and CCL5 in skin lesions in the cutaneous RPA reaction mouse model. We suggest that HMGB1 blockades may represent a new direction for the treatment of cutaneous vasculitis.
Highlights
Vasculitis, that can occur in all sizes and types of blood vessels in almost all organs, is a procedure of clinical pathology, characterized by an infiltration of inflammatory cytokines around the blood vessel wall and blood vessels
The anti-inflammatory effects of HMGB1 blockades stems from an understanding of the biological basis of the HMGB1 inflammation, and we further investigate the role of HMGB1 in the pathogenic mechanism of vasculitis in a mouse model of cutaneous reverse passive Arthus (RPA) reaction
Significant Improvement in Inflammatory Cells Infiltration, Vascular Fibroid Necrosis, and Vasodilation Treated With the HMGB1 Blockade
Summary
Vasculitis, that can occur in all sizes and types of blood vessels in almost all organs, is a procedure of clinical pathology, characterized by an infiltration of inflammatory cytokines around the blood vessel wall and blood vessels. With the absence of pathogens, endogenous molecules (e.g., HMGB1 is passively released from necrotic cells or secreted by stressed cells to respond to cellular injury) activate APCs, resulting in autoimmune diseases and transplant rejections [9, 10]. It has been proven in our previous study that HMGB1 is involved in the pathogenesis of inflammatory and autoimmune disorders, such as, Henoch– Schonlein purpura (HSP), allergic vasculitis (AV), and urticarial vasculitis (UV). The function and mechanism of HMGB1 in vasculitis has not been clearly stated
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
