Abstract
ABSTRACT Objectives：Achyranthes japonica (AJ) has been used as an anti-bacterial and anti-inflammatory agent . However, it is unclear that AJ could show the anti-inflammatory effects in macrophages. In this experiment, we studied whether AJ could inhibit the inflammatory responses in macrophages. Methods：To measure out the cytotoxicity of AJ, we performed the MTT ass ay. We evaluated the nitric oxide (NO) production, and cytokine production such as interleukin (IL)-1b, IL-6 and tumor necrosis factor (TNF)-a. We also investigated the cellular mechanims such as mitogen act ivated protein kinases (MAPK)s and nuclear factor kappa B (NF-kB).Results：AJ inhibited lipopolysaccharide (LPS)-induced NO production. AJ also inhibited production levels of IL-1b, IL-6 and TNF-a in LPS-stimulated macrophage. Finally, we stern blot analysis showed that AJ treatment inhibited the activation of p38 but not of extracellu lar signal-regulated kinase, c-jun NH2-terminal kinase and NF-kB. Conclusions：These results showed that AJ down-regulated the inflammatory response via p38 in macrophages, which suggest that AJ could be a candidate on treating inflamma tory diseases.Key words：Achyranthes japonica (AJ), cytokine, inflammation
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