The Antihypertensive Effect of Marchin-13 Tang on L-NAME-induced Hypertension in Rats

Abstract

Marchin-13 Tang is a traditional Mongolian medicine widely used to reduce blood pressure. The study aimed to evaluate the anti-hypertensive effect of Marchin-13 (M-13) Tang in the L-NAME-induced model of hypertension. The biological composition activity of M-13 Tang was examined using the UV spectrophotometric method. The experimental groups induced Hypertension models by L-NAME 40 mg/kg. The concentrations of serum nitric oxide (NO), Angiotensin-Converting Enzyme (ACE), superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) were measured in M-13 Tang treatment groups by enzyme-linked immunosorbent assay (ELISA). The content of total phenolics was measured at 2.96±0.16%, while flavonoids were found to be present at a level of 1.81±0.1%. The reductions in mean arterial pressure (MAP) were statistically significant. They were observed from day 14 to day 21 after giving M-13 Tang at 90 mg/kg and 180 mg/kg (p<0.01). Moreover, treated with M-13 Tang 90 mg/kg and 180 mg/kg groups, serum levels of NO, SOD, and CAT were significantly (p<0.01) increased compared with the L-NAME (40 mg/kg) group. The levels of MDA and ACE showed a significant decrease in both the Marchin-13 Tang-treated groups compared to the L-NAME group (p<0.05). The results of our study illustrate that Marchin-13 effectively reduced blood pressure by mitigating oxidative stress, enhancing NO production, and decreasing ACE levels in hypertensive rats induced by L-NAME.