Abstract

The aims of this study were to assess the distribution of Vibrio spp. isolated from cultured clams in Taiwan, investigate its resistance to drugs, and determine the expression of the b/a(subscript TEM) and b/a(subscript SHV) genes in the plasmid and/or chromosome. An analysis of the 205 Vibrio strains that were isolated from clams showed that V. alginolyticus (103/205) was the dominant species. The minimal inhibitory concentration (MIC) of these antibiotics was determined using the serial plate dilution method and the results indicated that most Vibrios were sensitive to oxolinic acid and least sensitive to amoxicillin. Seventy-seven Vibrios strains were highly resistant to amoxicillin. The resistance of amoxicillin is mostly due to the production of β-lactam ase, however, there were 12 β-lactamase negative Vibrios stains resistant to amoxicillin and clavulanic acid. An investigation of the b/a(subscript TEM) and b/a(subscript SHV) genes coding for β-lactamase in amoxicillin-resistant strains revealed that the b/a(subscript SHY) gene was located in the plasmid and chromosome, but the b/a(subscript TEM) gene was only found in the chromosome.

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