The Antibiotic Resistance of Bacterial Pathogens Isolated From Poultry Manure Used For Fish Ponds

Abstract

This study was carried out to isolate and identify antibiotic resistant bacteria from poultry manure usually used for pond fertilization. We collected poultry manure which used in Experimental Center for Fisheries Research, Tapize (ECFR), Agricultural University of Tirana in 2012. During the study we have isolated five bacterial pathogens; Salmonella typhi, Escherichia coli, Shigella dysenteriae, Staphylococcus aureus and Aeromonas hydrophila. Antibiotic susceptibility testing carried out using the disk diffusion technique. Antibiotics used were: enrofloxacin, amoxicillin, tetracycline, ampicillin, erythromycin and gentamicin. All the isolated organisms were 100% sensitive to enrofloxacin. The multiple resistance pattern revealed that 100% were resistant to tetracycline, 84% resistant to ampicillin, 81% resistant to amoxicillin, 66% resistant to gentamicin and 23% resistant to erythromycin. The risk posed by untreated poultry manure used in fish pond fertilization and the public health implications of these results were discussed. Keywords: Pathogenic bacteria, antibiotic resistance, sensitivity, poultry manure.