Abstract
The over-use of antibiotics has promoted multidrug resistance and decreased the efficacy of antibiotic therapy. Thus, it is still in great need to develop efficient treatment strategies to combat the bacteria infection. The antimicrobial photodynamic therapy (aPDT) and silver nanoparticles have been emerged as effective antibacterial methods. However, the silver therapy may induce serious damages to human cells at high concentrations and, the bare silver nanoparticles may rapidly aggregate, which would reduce the antibacterial efficacy. The encapsulation of sliver by nano-carrier is a promising way to avoid its aggregation and facilitates the co-delivery of drugs for combination therapy, which does not require high concentration of sliver to exert antibacterial efficacy. This work constructed a self-assembled supermolecular nano-carrier consisting of the photosensitizers (PSs), the anti-inflammatory agent and silver. The synthesized supermolecular nano-carrier produced reactive oxygen species (ROS) under the exposure of 620-nm laser. It exhibited satisfying biocompatibility in L02 cells. And, this nano-carrier showed excellent antibacterial efficacy in Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) as indicated by bacterial growth and colony formation. Its antibacterial performance is further validated by the bacteria morphology through the scanning electron microscope (SEM), showing severely damaged structures of bacteria. To summary, the supermolecular nano-carrier TCPP-MTX-Ag-NP combining the therapeutic effects of ROS and silver may serve as a novel strategy of treatment for bacterial infection.
Highlights
For treating the bacteria infection, antibiotics are the most widely used agents
The silver element analysis by atomic absorption spectrum (AAS) further demonstrated that nearly 100% of the Ag+ was successfully encapsulated into the TCPP-MTX-Ag-NP
The Ag-doping significantly changed the morphology of nanocarrier, which implied the successful Ag-doping in the TCPP-MTX-Ag-NP
Summary
For treating the bacteria infection, antibiotics are the most widely used agents. It is still in great need to develop efficient strategies to combat the bacteria infection. Photodynamic therapy (PDT) that utilizes photosensitizers (PSs) and light irradiation to generate cytotoxic reactive oxygen species (ROS) has been supposed to be a promising option in bacteria ablation (de Melo et al, 2013; Hamblin, 2016; Galstyan et al, 2017; Wainwright et al, 2017; Deng et al, 2019; Qiu et al, 2019; Zhang et al, 2020). Antimicrobial PDT (aPDT), named as photodynamic inactivation (PDI), uses visible light or near-infrared (NIR) light of appropriate wavelength to convert oxygen to cytotoxic ROS. As ROS can target numerous molecules, the microbes are very unlikely to develop resistance
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