The Antibacterial Activity of Gentamicin Sulphate in NaCl and Dextrose-NaCl Infusion against Escherichia coli Atcc 25922 and Staphylococcus aureus Atcc 29213

Abstract

Gentamicin sulphate mixed into the fluid infusion of NaCl and dextrose-NaCl is the process of intravenous admixtures, where knowledge about sterility, physicochemistry and stability properties of drugs, incompatible of drugs and the risk of exposure are matter to be considered. An already reconstituted drug has a time limit of stability and the long-time mixing will decrease its activity in inhibiting bacteria. The study aims was to determined the antibacterial effect of the infusion of 0.9% NaCl and 5% dextrose-0.225% NaCl against Escherichia coli and Staphylococcus aureus, the influence of time variation of the use of gentamicin sulphate injection, and the effect of interaction of infusion and time of use towards its activity on bacteria. The laboratory experiment research method was done, which included mixing sterile preparation of gentamicin sulphate injection into NaCl and dextrose-NaCl infusion in LAF, tested antibacterial activity against Escherichia coli and Staphylococcus aureus and data analyzed with ANOVA. The results showed that the type of fluid infusion was only influential as antibacterial activity against Staphylococcus aureus but not against Escherichia coli. On the other hand, the use of time variation was effective on gentamicin sulphate activity against both bacteria. Also, the interaction of fluid infusion with time of use has no effect on its activity on bacteria.