Abstract
Antibacterial compounds are generally produced from many herbal ingredients, but antibacterials may be produced from soil microbes, one of which is Actinomycetes. Actinomycetes are a soil bacterium capable of producing secondary metabolites in the form of antibacterial compounds. The antibacterial compound has the potential as an alternative antibacterial agent against oral microbes. One of the oral microbes that have a major role in the occurrence of caries isStreptococcus mutansandLactobacillus acidophilus. This study aimed to analyze the antibacterial activity of Actinomycetes against the growth ofS. mutansandL. acidophilusActinomycetes extracts at the concentration of 0.625%, 1.25%, 2.5%, 5%, 10%, 20%, and 40% were respectively exerted as the treatment groups while 0.12% Chlorhexidine Gluconate (CHX) and aquadest as control. The measurement of antibacterial activity is done by looking at MIC and MBC. One Way ANOVA and Post Hoc Dunnets T3 test MIC and MBC values show p=0,000 (p <0.05). Actinomycetes extract at the concentration of 0.625% was able to inhibit the growth ofS. mutansandL. acidophiluswhich was determined as the MIC and at the concentration of 40% as MBC of S.mutans and the concentration of 10% as MBC ofL. acidophilus. There was an antibacterial activity of Actinomycetes to the growth ofS. mutansandL.acidophilus.
Highlights
Indonesia is one of the countries having the most extensive peatlands compared to other tropical countries, which is around 21 million ha, mainly spread to the islands of Kalimantan, Sumatra, and Papua
The results of antibacterial activity tests were obtained by measuring the difference between the absorbance values in anaerobic conditions
The result of this Antibacterial Activity of Actinomycetes on The Growth of Streptococcus mutans and Lactobacillus acidophilus study showed that Actinomycetes extracts with concentrations of 0.625%, 1.25%, 2.5%, 5%, 10%, 20%, and 40% could inhibit growth (MIC) of Streptococcus mutans and Lactobacillus acidophilus, starting from a concentration of 0.625%
Summary
Indonesia is one of the countries having the most extensive peatlands compared to other tropical countries, which is around 21 million ha, mainly spread to the islands of Kalimantan, Sumatra, and Papua. Peatland is an ecosystem included as wetlands This type of land was formed due to the accumulation of organic material over a long period, compared to the slow rate of decomposing organic matter in the wet or inundated parts of the forest. This process of organic materials, decomposition, or breakdown can occur without the role of microbes [1]. Rante et al (2017) studied the Actinomycetes isolated from the soil rhizosphere. They examined the effect of Actinomycetes extract with a concentration of 5%, 10%, and 20% to Staphylococcus aureus. Staphylococcus aureus is a normal flora inside the mouth cavity [4]
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