The Antibacterial Activity of a Fraction Extracted from Camel Urine against Mycobacterium tuberculosis Isolated from Tuberculosis Patients in Jeddah City

Abstract

Tuberculosis (TB) is a disease caused by aerobic bacteria called Mycobacterium tuberculosis. The influx of workers from various regions of Southeast Asia and the Indian sub-continent, who come to work in Saudi Arabia, is one of the major important reasons for the spread of the disease. PMF (Prophet Medicine fraction), is a biologically active fraction isolated from dried camel urine (PM701) by Prof. Fatin Khorshid (patented), it has many advantages as an antimicrobial agent against fungi species and Gram-positive bacteria and two Gram-negative bacteria. The aim of this study to investigate the effect of PMF (Prophet Medicine fraction) an extraction from camel urine against Mycobacterium tuberculosis bacteria (MTB) that causes Tuberculosis (TB). In addition, evaluate the period of treatment required to kill the bacteria, and comparing it with Rifampicin (RIF) antibiotic. This study depends on an experimental design that uses 10 random samples of TB patients from King Fahad Armed Hospital (KFAH) Processed. Each sample were cultured with different PMF concentrations using BACTEC 960 culture system and measurement by GeneXpert MTB/RIF test. Interpretation of 10 samples showed no effects of PMF drug compared with the RIF effect, only 10% concentration. Showed some effect. In the first test, there was an increasing number of MTB with all different concentrations of PMF that including 5%, 7.5%, 12.5%, and 15%. Similarly, all these samples are moved to the GeneXpert MTB/RIF test, and which showed that there are no effects in all PMF concentrations except 10%. Therefore, could conclude from these results that there is a non-significant effect of the PMF concentrations against MTB the 10% which probably be the ideal concentration of this drug that reduces the number of bacteria, evidenced by the P-value of the F test less than 0.05 %. In this research, it was predicted that the PMF substrate would work as an antibiotic agent only with certain conc., which has many advantages such as; it is cheap, easier to find in Arab areas and it is non-toxic. The results showed no complete effect comparison between growth control of MTB treated with RIF and MTB treated with PMF except 10% that need further studies. Keywords: Camel urine, Mycobacterium tuberculosis, PMF, Rifampicin.