Abstract
In vitro chemical studies have shown that natural anti‐oxidants are beneficial compounds that prevent and may help repair DNA damage caused by intracellular reactive oxygen species (ROS), such as hydrogen peroxide. Thiols (RSH), naturally‐occurring, electron‐rich, biological compounds, donate electrons to neutralize free radicals, removing them from the body. Here we seek to elucidate the biochemical pathway by which the thiol cysteamine protects against oxidation by hydrogen peroxide in the absence of endogenous oxidative stress or DNA repair machinery. Previous investigations have shown that Saccharomyces cerevisiae gene deletion strains lacking the ctt1 gene exhibit increased growth in the presence of cysteamine as compared to their untreated counterparts when assaulted with hydrogen peroxide. The current study investigates the potential protective effects of cysteamine in other S. cerevisiae knock‐out strains. Western Blot analysis is used to test protein levels in the oxidative response and DNA repair pathways in the presence and absence of cysteamine.
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