The Anti-microbial Activity of Polyherbal Extract using Escherichia Coli and Saccharomyces Cerevisiae

Abstract

One of the biggest issues in contemporary life is antimicrobial resistance. Therefore, a remedy to this issue is required. Combinations of various antimicrobial medications ought to be more effective than single medications. The usual plants that grow in our area have antibacterial properties. One of the main plants with strong antibacterial action is Azadirachta indica. Eryngium foetidum and Mimosa pudica both have demonstrated antibacterial action and are readily available. The combination of these three herbal plants extracts ought to provide stronger antibacterial effects than any of the plants separate extracts. Raw plants are initially gathered from the area. The Azadirachta indica, Eryngium foetidum, and Mimosa pudica leaves and roots are employed. To get rid of dirt and undesired detritus, plants are thoroughly washed and dried in the shade. The plant leaves are then thoroughly pulverized individually. Then, using a Soxhlet device and ethanol the powdered plants are extracted separately. The extraction process is carried out until a clear solution is found. The ethanol is then removed from the obtained extracts by evaporation. DMF (dimethyl formamide) is used to dilute the extract so that 1000mcg, 2000mcg, and 3000mcg of each plant extract and plant extract mixture is also prepared in three different concentrations. The Disc Plate method, which employs Saccharomyces cerevisiae and E. coli, is used for antimicrobial assay. It is decided to use nutrient agar for bacteria and sabouraud dextrose agar for fungi. The prepared substance discs dipped in plant extracts and a combination are then added to the inoculation medium in an aseptic space with a laminar airflow system after it has been first inoculated with the organisms. The plates are then left to incubate for 24 hours. The outcomes were as anticipated; the plant extract combo displayed a greater zone of inhibition than the plant extracts taken separately. It measures the zone of inhibition.