The Anti-Inflammatory and Antioxidative Effects of Ningmitai Capsule in the Experimental Autoimmune Prostatitis Rat Model.

Haicheng Chen,Xiangzhou Sun,Yun Xie,Jiahui Yao,Linyan Lv,Chunhua Deng,Guihua Liu,Cuncan Deng,Chi Zhang

doi:10.1155/2020/5847806

Abstract

Objective Ningmitai (NMT) capsule has been widely prescribed for the treatment of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), but the mechanism remains unclear. This study aims to evaluate the therapeutic effects of the NMT capsule in the experimental autoimmune prostatitis (EAP) rat models and explore its possible mechanisms. Methods A total of fifty male Sprague Dawley rats were used in this study. Prostate extract was obtained for the induction of EAP rat models. The EAP rats were randomly divided into the model group, NMT low-dose group (0.45 g/kg/d), NMT medium-dose group (0.90 g/kg/d), and NMT high-dose group (1.80 g/kg/d), with six rats per group. Three NMT treatment groups were administered with the NMT capsule by gavage for 30 days. HE staining was used for histopathological analyses of prostate tissues. Western blotting was used to measure the expression of proinflammatory factors IL-1β and TNF-α. The MDA level was detected to reflect the level of oxidative stress. The bilateral dorsal root ganglia of T3/L1 to S4 were dissected to measure the substance P expression. Results EAP rat models were successfully constructed, in which extensive infiltration of inflammatory cells was found. Treatment of NMT capsule for 30 days and the infiltration of inflammatory cells were significantly mitigated (P < 0.05), especially in the NMT medium-dose group and NMT high-dose group. Moreover, the expression of IL-1β and the level of MDA were significantly decreased (P < 0.05). In addition, NMT treatment could significantly alleviate substance P expression in dorsal root ganglia. Conclusion Our findings demonstrate that the NMT capsule can alleviate inflammatory response and oxidative stress and reduce the production of substance P in EAP rats. This provides a theoretical basis for the clinical application of NMT capsule for CP/CPPS treatment.

Highlights

Chronic prostatitis/chronic pelvic pain syndrome (CP/ CPPS) is defined as chronic pelvic pain not caused by other identifiable pathology, according to the classification of prostatitis developed by the National Institutes of Health (NIH) in 1994 [1]
We aim to evaluate the therapeutic effects of the NMT capsule and explore its possible mechanisms to alleviate CP/CPPS in the experimental autoimmune prostatitis rat model
Ese results suggest that prostatitis in rats would cause the loss of body weight, and the treatment with NMT capsule is beneficial to restore the weight in rats

Summary

Introduction

Chronic prostatitis/chronic pelvic pain syndrome (CP/ CPPS) is defined as chronic pelvic pain not caused by other identifiable pathology, according to the classification of prostatitis developed by the National Institutes of Health (NIH) in 1994 [1]. It is a common disease in adult men and affects the quality of patients’ life. Antibiotics and α-blockers have been widely used for the management of CP/CPPS [3], the efficacy of these treatments is usually unsatisfactory in clinical practice. Doctors usually choose antibiotics to treat CP/CPPS according to experience, so that the efficacy of antibiotics is doomed to be limited. Several RCTs research studies showed heterogeneous outcomes of monotherapy with α-blockers [4,5,6,7]. ese results suggested that α-blockers may not be recommended as a first-line monotherapy [8]

Methods

Results

Conclusion