The anti-inflammation effect of Baige capsule and its principal components mixture in MCAO rats

Abstract

Background: Baige (BG) is a compound Chinese herbal preparation, constituted of different position extracts (ethanol extracts from Pueraria lobate and SFE-CO2 extracts from Radix Angelicae dahuricae) of P. lobata and A. dahurica to treat the brain injury in patients.Aim: The goal of this study was to identify the neuroprotective properties of BG and its principal component mixture (PCM) and verify whether the material basis for BG is its PCM.Methods: Middle cerebral artery occlusion (MCAO) was operated on male Sprague-Dawley rat for 2 h, different doses of BG or PCM or vehicle were gavaged after 3 h of MCAO. Rats were sacrificed after 30 days treatment. Blood serum inflammation factors and NGF were detected by ELISA.Results: After 30 days of treatment, both BG and PCM interventions reduced the infarct volume, modified neurological severity score (mNSS) in rats, declined IL-1β and IL-6 levels in the serum, increased NGF level in the serum and recovered the number of Nissl body in injured brain.Conclusions: Both BG and PCM exert equivalent levels of recovery effect in MCAO on rats; and PCM is the material foundation of BG. This recovery effect is associated with inflammatory inhibition and NGF production.