Abstract
The anti-aging activity of many plant flavonoids, as well as their mechanisms of action, have been explored in the current literature. However, the studies on the synergistic effects between the different flavonoid compounds were quite limited in previous reports. In this study, by using a high throughput assay, we tested the synergistic effects between different citrus flavonoids throughout the yeast’s chronological lifespan (CLS). We studied the effect of four flavonoid compounds including naringin, hesperedin, hesperitin, neohesperidin, as well as their different combinations on the CLS of the yeast strain BY4742. Their ROS scavenging ability, in vitro antioxidant activity and the influence on the extracellular pH were also tested. The results showed that neohesperidin extended the yeast’s CLS in a concentration-dependent manner. Especially, we found that neohesperidin showed great potential in extending CLS of budding yeast individually or synergistically with hesperetin. The neohesperidin exhibited the strongest function in decreasing the reactive oxygen species (ROS) accumulation in yeast. These findings clearly indicated that neohesperidin is potentially an anti-aging citrus flavonoid, and its synergistic effect with other flavonoids on yeast’s CLS will be an interesting subject for future research of the anti-aging function of citrus fruits.
Highlights
Aging, a complex and multifactorial biological process, can be defined as a gradual loss of physiological and psychological integrity, leading to gradual deterioration in almost all functions and the increased vulnerability to death [1]
dimethyl sulfoxide (DMSO) was universally used as a solvent for the water-insoluble drugs, and different concentrations of DMSO variably affected the growth of the yeast [23]
0.2% and 0.4% DMSO in the culture medium when compared with the control, while the lifespan at
Summary
A complex and multifactorial biological process, can be defined as a gradual loss of physiological and psychological integrity, leading to gradual deterioration in almost all functions and the increased vulnerability to death [1]. A treatment that targets the multiple factors and/or pathways within the aging process is good candidate for study. The trend of population aging is gradually increasing. Because of this trend in population, it is of great practical significance to find effective ways to slow aging or improve the healthy state of aging. The anti-aging activity of Molecules 2019, 24, 4093; doi:10.3390/molecules24224093 www.mdpi.com/journal/molecules. Many different plant compounds have been suggested to have direct/potential anti-aging activity in the existing literature [2,3,4,5]
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