The antagonistic effect of magnesium-selenium against fluorosis on the expressions of amelogenin in dental germ of mice incisor

Abstract

Objective To study the antagonistic effects of magnesium- selenium- fluorine preparation on dental fluorosis of mice and its mechanism, and to provide a foundational basis for prevention and control of dental fluorosis. Methods Eighty male SPF ICR mice, were divided into 8 groups according to body weight by random number table method: control group, magnesium group, selenium group, magnesium-selenium group, fluoride group, magnesium-fluorine group, selenium-fluorine group and magnesium-selenium-fluorine group. The control group, magnesium group, selenium group and magnesium-selenium group drank double steamed water, and the other four groups drank 50 mg/L F- double steamed water. The control group and fluoride group fed conventionally. Magnesium group and magnesium-fluorine group fed conventionally by adding MgSO4·7H2O 162.5 mg/kg. Selenium group and selenium-fluorine group fed conventionally by adding Na2SeO3·5H2O 2.0 mg/kg. Magnesium-selenium group and magnesium-selenium-fluorine group fed conventionally by adding MgSO4·7H2O 162.5 mg/kg and Na2SeO3·5H2O 2.0 mg/kg. Then incisor specimens were obtained after the mice were put into death after treatment for 42 days. The expression of amelogenin was observed with immunohistochemical staining. And gray value is expressed as a result, the greater the gray value, the less protein expression. Results The results of light microscope showed that ameloblasts in the fluoride group showed disarrangement and even had vacuolar. The change of ameloblasts in the magnesium-selenium-fluorine group had no significant difference with control group. The expressions of amelogenin in enamel matrix of fluoride group (131.03 ± 11.14) was significantly higher than those of others (control group: 143.44 ± 2.52, magnesium group: 143.73 ± 12.43, selenium group: 148.89 ± 2.85, magnesium-selenium group: 148.38 ± 7.58, magnesium-fluorine group: 145.90 ± 7.00, selenium-fluorine group: 148.70 ± 4.90, and magnesium- selenium- fluorine group: 151.89 ± 4.59, allP 0.05). The fluoride, magnesium and selenium affected the expression of amelogenin in ameloblast (F = 18.463, 9.372, 4.741, allP 0.05). Conclusions There is an antagonistic effect of magnesium on the expression of enamel protein in fluorosis mice. However, it can not be considered that there is an effect of selenium on the expression of enamel protein in fluorosis mice. Key words: Fluorine; Magnesium; Selenium; Amelogenin