Abstract
A new sensitive DNA probe containing cetylpyridinium bromide (CPB) and emodin (an effective component of Chinese herbal medicine) was developed using the resonance Rayleigh light scattering (RLS) technique. A novel assay was first developed to detect DNA at nanogram level based on the ternary system of DNA–CPB–emodin. The RLS signal of DNA was enhanced remarkably in the presence of emodin–CPB, and the enhanced RLS intensity at 340.0nm was in direct proportion to DNA concentration in the range of 0.01–2.72μgmL−1 with a good linear relationship. The detection limit was 1.5ngmL−1. Three synthetic DNA samples were measured obtaining satisfactory results, the recovery was 97.6–107.3%.
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