Abstract
Abstract High-resolution two-dimensional polyacrylamide gel electrophoresis (2D PAGE) is a technique which separates a mixture of proteins according to their intrinsic charge properties by isoelectric focusing (IEF) in the first dimension and according to their molecular size by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension (1). This technique is capable of resolving simultaneously complex mixtures of proteins containing several thousand components, and makes 2D PAGE a powerful tool in many areas of biological and biomedical research (2). It results in complex two-dimensional protein profiles from which only a limited amount of information can be extracted by simple visual inspection.
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