The Analysis of the Mycobacterium Leprae Resistance Against Ofloxacin After the Rifampicin Ofloxacin Minocycline (ROM) Therapy

Abstract

Background: This reserach aimed to determine the correlation between the morphology index and IgM anti PGL-1, and the mutations in gyrA genes in order to detect ofloxacin resistance in the patients with multibacillary (MB) leprosy. Methods: This research was conducted in Dr. Wahidin Sudirohusodo General Hospital and its network hospitals in Makassar using the cohort retrospective design. The research sample comprised 21 patients with MB leprosy who had completed the ROM treatment regimens, and whose bacterial index (BI), morphology index (MI), IgM anti PGL-1 antibody, PCR, and sequencing, skin lesion number, and treatment regularity had been examined. Result: The research revealed that all the samples did not undergo any mutations of the gyrA genes which was responsible for the ofloxacin resistance; however, there was a correlation between BI and the IgM anti PGL-1 antibody, and between IgM anti PGL-1 antibody and the number of the skin lesions. Conclusions: There was no samples found to undergo the mutations of gyrA genes, this could be used as the reference to assess the correlation between the morphology index and the IgM anti PGL-1 antibody with the mutations of the gyrA genes.