The analysis of the 4 enzymatic assays in enzyme RELA results

Abstract

Objective To reflect the capability of the Chinese reference laboratories through the assessment and analysis of the uniformity and uncertainty for GGT, LDH, ALT and AST among RELA from 2006 to 2011. Methods To collect the measurement results and the combined measurement uncertainties of all laboratories that had participated in RELA during 2006 and 2011.The testing results and uncertainties of the four enzymes among RELA were grouping analyzed. They were divided into four groups：the whole group (all laboratories, 12-27 labs annual), the foreign group (laboratories except for the Chinese laboratories, 4-10 labs annual), the Chinese group (all Chinese laboratories, 6-18 labs annual) and the reference group (LAB 3). Calculate the CVs of inner-group measurement results, the bias of the Chinese group and the reference group measurement results to the foreign group and the elative standard measurement uncertainties, and evaluate the uniformity of measurement results and the ur variation trends. Results There were annual downtrend for coefficient of variation except LDH. Except ALT in 2006, CVs of the foreign group, the Chinese group and the whole group were all below 5%. The Chinese group had 87.5%（42/48）smaller CV than the foreign group in the last 6 years. The biases of the Chinese group to the foreign group were from -2% to 2% except GGT and ALT in 2006 and ALT in 2008. The Chinese group had 66.7% results less than the foreign group during 2006 and 2011. There was significant differences between the Chinese group measurement results (3.525 μkat/L) and the foreign group (3.647 μkat/L) of ALT high batch in 2008 (T=2.635，P<0.05). The relative standard measurement uncertainties of the four groups had the same uptrend and the low batch was 10% higher than the high batch. The reference group had a stable ur of 1.2%. The Chinese group had a lower ur than the foreign group during 2007 and 2009, but had similar levels after 2010. The Chinese group had constant relative standard uncertainties of 1.0% in 2009, 2010 and 2011. Conclusions There was no significant differences between the Chinese group and the foreign group, and they had good uniformity .The results of the relative standard measurement uncertainty were objective and stable.（Chin J Lab Med,2014,37:347-351） Key words: Gamma-Glutamyltransferase; Lactate dehydrogenases; Alanine transaminase; Aspartate aminotransferases; Reproducibility of results