Abstract
A method of analysis is described which is applicable to solutions of epinephrine and norepinephrine, such as epinephrine injection U. S. P. and levarterenol bitartrate injection N. N. R. The method depends on the quantitative conversion of the substances into O3, O4, N‐triacetyl derivatives which may be extracted into chloroform, weighed, identified, and examined polarimetrically for the determination of stereo‐chemical composition. Excellent recoveries of the physiologically potent l‐isomer are obtained, and results of analyses of solutions of epinephrine show very good agreement with potencies determined by the U. S. P. XIV dog bioassay. By supplementing the method with partition chromatography of the acetylation product, the norepinephrine in a solution of U. S. P. epinephrine may be quantitatively isolated as its triacetyl derivative and determined colorimetrically after deacetylation and oxidation to noradrenochrome.
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