The Analysis of Selenoprotein and Se-Polysaccharide in Selenium-Enriched <i>Lentinan edodes</i>

Abstract

The cultivation of Lentinan edodes as a carrier for Se, low concentrations of sodium selenite solution were added directly to the medium to inorganic selenium be absorbed into the mushroom body, and selenium-enriched Lentinan edodes can be got. Using selenium-enriched mushrooms as raw material, it was preliminarily studied that patterns of occurrence of selenium and the contents of Selenoprotein and Se-polysaccharide in their fruiting bodies of mushrooms. Various extraction solvents were used to distill and separation Se-polysaccharide and Se-protein of the Lentinan edodes, and atomic absorption spectrometer was used to determine the selenium contents of all component parts. The results showed that Lentinan edodes have the ability of enrichment selenium and can transform inorganic selenium in the environment into organic selenium. The main group of Selenium is organic state. The selenium in protein occupied the total selenium 40.09%, In the four components of selenium protein, water-soluble proteins of selenium is main (21.65% of the total selenium).The selenium in polysaccharide occupied the total selenium 27.83%. Mass fraction of acid-soluble Se-polysaccharide than in water-soluble Se-polysaccharide and alkali-soluble Se-polysaccharide is higher (13.52% of total selenium). The main distributed form of the selenium is from the organic matter in the analyzed Lentinan edodes, the combined selenium in protein was higher.