Abstract
Some examples are described in which the molecular cloning of plant DNA and the associated techniques of molecular biology have already contributed to plant genetics and plant breeding research. They include ( a ) the isolation and determination of the complete sequence of some nuclear genes, ( b ) the detection of genetic variation in gene structure and copy number, ( c ) the physical mapping of the chloroplast genome, ( d ) the physical mapping and identification of nuclear chromosomes by in situ hybridization, ( e ) a molecular description of highly repeated sequences in heterochromatin, ( f ) a rapid method for classifying cytoplasmic variants of maize and ( g ) characterization of mitochondrial DNA sequences necessary for normal pollen development in maize. It is emphasized that the mapping of genomes by using molecular methods does not require genetic variation or expression of the genes in the phenotype. Where a nucleic acid probe is available for a gene, then recessive allelic variants or inactive genes can be detected that do not contribute to the phenotype.
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Schedule a callMore From: Philosophical Transactions of the Royal Society of London. B, Biological Sciences
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