Abstract
The behaviour of several sterols and related compounds on chromatoplates prepared from kieselgur G has been studied. Using a cyclohexane-ethyl acetate mixture (99.5:0.5), the separation of some sterols, e.g. ergosterol-cholesterol, cholesterol-lanosterol, cholesterol-vitamin D 2 has been achieved. In the reversed-phase system: undecane/acetic acid-water several sterols or their corresponding acetates can be fractionated. In this way cholesterol acetate and the acetates of the major phytosterols, viz. β-sitosterol and stigmasterol, are clearly separated, thus enabling the analysis of mixtures of vegetable and animal fats. By adding bromine to the mobile phase a differentiation of the critical pair of sterols cholesterol-brassicasterol has been accomplished.
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