The analysis of germination of two types of <em>Momordica charantia </em>L. seeds in different media after different storage durations

Abstract

Momordica charantia L. is a member of Cucurbitaceae can be use as rootstock for obtain seedlings againsts some abiotic and biotic stress factors. For these reasons, the aim of this study was to evaluate effect of origin of the plant types, germination media and seed storage duration on seed germination and seedling rates. The two different plant types (A and B) belonging to non-commercial gardens from two different districts located 30 km from each other in Kocaeli City were tested in 3 seed germination media (paper towel, petri dishes and sand) after 0, 90, 180 and 270 days storage under dark-room condition. First and last germination days, first and last leaf appearance days, germination and seedling rate were monitored. The study designed as Completely Randomized Design replicated thrice and after statistical analyzes, analysis of variance with Duncan Multiple Range Test method at P˂0.05 was performed. Considering the whole experiment first germination days changed between 4th-5.7th and last germination days changed between 4th-13.7th days. There is no difference between the plant types in the parameter at all the three media. Maximum germination and seedling ratio were observed at type-A in the all media. In petri, the germination and seedling ratio parameter decreased gradually with storage duration although storage duration showed no differences in paper towel or sand in the germination and seedling parameters. Optimum seedling ratio of Momordica charantia was observed when sand media were used at ratio of 99.2% in type-A and 88.35% in type-B in averages at all the storage durations under the study condition.