Abstract
Dynamic sieving electrophoresis (DSE) is a new analytical tool used in capillary electrophoresis for the analysis of nucleic acids. By combining many of the principles of traditional slab gel methods with the use of entangled polymer solutions in surface-modified, thin-walled, fused-silica capillaries, it is possible to perform molecular size determinations of DNA restriction fragments and polymerase chain reaction (PCR) products. Analysis of DNA restriction fragments and PCR products by DSE was performed using a DB-17 surface-modified capillary and an entangled polymer solution of either hydroxyethyl cellulose or hydroxypropyl methylcellulose. DNA restriction fragments and PCR products were monitored at 260 nm. DSE has been shown to have higher resolution and efficiency than traditional slab gel methods. In an automated format, DSE offers speed, resolution, quantitation, and efficiency not available with current slab gel methods.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
