The Analysis of Cu(II)/Zn(II) Cyclopeptide System as Potential Cu,ZnSOD Mimic Center

Aleksandra Kotynia,Tomasz Janek,Żaneta Czyżnikowska,Justyna Brasuń,Sylwia Bielińska,Wojciech Kamysz

doi:10.1007/s10989-017-9574-8

Aleksandra Kotynia, Tomasz Janek + Show 4 more

Open Access

https://doi.org/10.1007/s10989-017-9574-8

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Abstract

In this paper are presented the features of copper (II) and zinc (II) heteronuclear complexes of the cyclic peptide—c(HKHGPG)2. The coordination properties of ligand were studied by potentiometric, UV–Vis and CD spectroscopic methods. These experiments were carried out in aqueous solutions at 298 K depending on pH. It turned out that in a physiological pH dominates Cu(II)/Zn(II) complex ([CuZnL]4+) which could mimic the active center of superoxide dismutase (Cu,ZnSOD). In next step we performed in vitro research on Cu,ZnSOD activity for [CuZnL]4+ complex existing in 7.4 pH by the method of reduction of nitroblue tetrazolium (NBT). Also mono- and di-nuclear copper (II) complexes of this ligand were examined. The ability of inhibition free radical reaction were compared for all complexes. The results of these studies show that Cu(II) mono-, di-nuclear and Cu(II)/Zn(II) complexes becoming to new promising synthetic superoxide dismutase mimetics, and should be considered for further biological assays.

Highlights

In recent years there has been observed a rapid expansion in the development of Cu(II) complexes as important bioactive compounds as potential drugs for therapeutic intervention in various diseases
In the present manuscript we show the results of potentiometric and spectroscopic studies for the Cu(II)/ c(HKHGPG)2/Zn(II) system
In this study we presented preliminary results describing the ability of the cyclic peptide c(HKHGPG)2 to form Cu(II)/ Zn(II) complexes depending on pH

Summary

Introduction

In recent years there has been observed a rapid expansion in the development of Cu(II) complexes as important bioactive compounds as potential drugs for therapeutic intervention in various diseases. A wide role of transition metal ions in biological processes induces the intensive development of studies allowing to the characteristic of copper homeostasis. These studies are performed by the using of the biomimetics mimicking the structure or activity of the naturally occurring systems. This interaction is responsible for the activity of number of metalloperoteins or their toxicity

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