Abstract
Hydrazine is rarely found as an intermediate in microbial nitrogen conversions. In this study the conversion of hydrazine by the anaerobic ammonium oxidation (Anammox) culture, in which hydrazine has been proposed as an intermediate, was investigated. This study demonstrated the biological nature of hydrazine conversion by the Anammox culture. In batch cultures with hydrazine it was observed that 3 mol N 2H 4 was disproportionated to 4 mol NH + 4 and 1 mol N 2. Hydrazine with nitrite as an electron acceptor showed a conversion of 3 mol N 2H 4 and 4 mol NO − 2 to 5 mol N 2, with a specific activity of 5.5 nmol min −1 (mg volatile suspended solids) −1. Addition of hydrazine to a biofilm reactor for 80 days showed that it was not possible to grow Anammox with hydrazine.
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