Abstract
Amylolytic actinobacteria were isolated from the rumen fluid of ruminants. Eighteen isolates actinobacteria were obtained and the generation of actinobacteria mutants using chemical agent Sodium Azide. The actinobacteria wild type and mutants were then screened for amylase activity using starch agar plate assay. Ten isolates showed the ability to produce amylase enzymes. Submerged fermentation (SmF) was used for microbial production of amylase. It was found that maximum amylase activity (2.53 U/mL) was produced by actinobacteria isolate R-9 M, and 2.43 U/mL was produced by isolate R-2 WT using pollard as a substrate in Submerged fermentation. This preliminary study could provide base information for the discovery of novel actinobacteria from natural resources such as rumen fluid for the production of amylase, which will be used for multipurpose.
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