The amino acid profile of yeasts from ketchup factory waste as a candidate of single cell protein (SCP)

Abstract

Yeast can be used as a feed additive to increase livestock health in the form of probiotic and immunostimulant. This research aims to observe the protein content and amino acid profile of yeast isolated from ketchup factory waste in East Java, Indonesia. The yeast biomass production was carried out in yeast malt broth medium containing 3% glucose, 0.3% malt extract, 0.5% bacto peptone and 0.3% yeast extract. Protein content in each yeast isolate was measured using Bradford method and the amino acid profile was estimated using High Performance Liquid Chromatography (HPLC). From seven yeast isolates detection resulted the highest protein content was KYP3K2 isolate containing 1172.33 µg/ml of protein. Besides, both isolates KYP3K2 and AYP6K2 have low nucleic acid content of 15.8 %. Amino acid profile showed that both samples consist of nine essentials amino acid and seven non essentials amino acid. In conclution, both samples fully recommended as Single Cell Protein as feed livestock.