Abstract
Salt stress affects the plant quality, which affects the productivity of plants and the quality of water storage. In a recent study, we conducted the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) analysis and RNA-Seq, bioinformatics study methods, and detection of the key genes with qRT-PCR. Our findings suggested that the optimum salt treatment conditions are 200 mM and 19d for the identification of salt tolerance in tomato. Based on the RNA-Seq, we found 17 amino acid metabolic and 17 carbohydrate metabolic pathways enriched in the biological metabolism during the response to salt stress in tomato. We found 7 amino acid metabolic and 6 carbohydrate metabolic pathways that were significantly enriched in the adaption to salt stress. Moreover, we screened 17 and 19 key genes in 7 amino acid metabolic and 6 carbohydrate metabolic pathways respectively. We chose some of the key genes for verifying by qRT-PCR. The results showed that the expression of these genes was the same as that of RNA-seq. We found that these significant pathways and vital genes occupy an important roles in a whole process of adaptation to salt stress. These results provide valuable information, improve the ability to resist pressure, and improve the quality of the plant.
Highlights
Tomato is an important and nutritious vegetable crop worldwide
Based on a correlation analysis (Table 1), all of the indicators are strongly correlated with the salt concentration, suggesting effective indicators that represent the salt tolerance of tomato
The TOPSIS analysis revealed the rank of Rj considering all indicator values for different salt concentrations during the response to salt stress (Table 2); 200 mM was the optimum NaCl concentration for the identification of salt tolerance
Summary
Tomato is an important and nutritious vegetable crop worldwide. Cultivated tomatoes are susceptible under a wide range of environmental pressures (Zhu et al, 2014; Zushi et al, 2014; Sasidharan and Voesenek, 2015). Salt stress can adversely affect the growth and productivity of plants. In the course of growth and development, plants have developed many physiological and biochemical mechanisms to adapt to the stress of the environment (Zhu et al, 2014), and a large number of physiological and metabolic reactions are produced in plants to accommodate this change (Yamaguchi-Shinozaki and Shinozaki, 2006; Hirayama and Shinozaki, 2010; Lata and Prasad, 2011; Tang et al, 2012). By studying the patterns of gene expression in different stress conditions, the adaptive mechanism of different stresses was analyzed (Zhu, 2002)
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